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Part:BBa_K3076213:Design

Designed by: Choi Jonas Sze Ngo   Group: iGEM19_Hong_Kong_JSS   (2019-10-08)


dsDNA substrate with KanR gene for CopA knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

The genomic sequence of CopA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.

References